Journal: EMBO Reports

Article Title: Apoptosis inhibitor 5 is an endogenous inhibitor of caspase‐2

doi: 10.15252/embr.201643744

Figure Lengend Snippet: Wild‐type or caspase‐3/7 double KO MEFs were treated with valinomycin (30 μM) for 24 h. Cells were then collected and treated with FAM‐VDVAD‐FMK‐FLICA reagent. Caspase‐2 activity was measured by FACS analysis following the manufacturer's protocol (ImmunoChemistry). Shown are data from a single representative experiment.

Article Snippet: The following primary antibodies were employed: anti‐caspase‐2 (clone 11B4, Alexis), anti‐API5, anti‐caspase‐9, anti‐PARP, anti‐His (Cell Signaling Technologies) and anti‐actin (Sigma).

Techniques: Activity Assay

Journal: BMC Cancer

Article Title: Apoptosis inhibitor-5 overexpression is associated with tumor progression and poor prognosis in patients with cervical cancer

doi: 10.1186/1471-2407-14-545

Figure Lengend Snippet: API5 and pERK1/2 expression in human cervical neoplasias specimens. (A) Representative immunohistochemical staining images of API5 and pERK1/2 in normal, low grade CIN, high grade CIN, and cervical cancer tissues. Bars: 100 μm. (B) API5 IHC staining score in cervical neoplasia samples. API5 IHC staining score in cervical cancer samples was significantly higher than that of all other groups. The mean API5 score associated directly with each tumor stage, stage I tumors stained more weakly than stage II and stage IV tumors. API5 IHC staining score in poorly differentiated cervical cancer samples was significantly higher than that of well/moderately differentiated cancers.

Article Snippet: The slides were then stained with anti-API5 mouse monoclonal antibody (Sigma-Aldrich, clone no. 1C2, 1:250 for 2 hr at room temperature) and rabbit anti-pERK1/2 monoclonal antibody (Cell Signaling, Danvers, MA; clone no. 20G11, 1:150 for 2 hr at room temperature) in a Dako Autostainer Plus (Dako).

Techniques: Expressing, Immunohistochemical staining, Staining, Immunohistochemistry

Journal: BMC Cancer

Article Title: Apoptosis inhibitor-5 overexpression is associated with tumor progression and poor prognosis in patients with cervical cancer

doi: 10.1186/1471-2407-14-545

Figure Lengend Snippet: Expression of API5 and pERK1/2 in relation to clinicopathologic characteristics in IHC analysis

Article Snippet: The slides were then stained with anti-API5 mouse monoclonal antibody (Sigma-Aldrich, clone no. 1C2, 1:250 for 2 hr at room temperature) and rabbit anti-pERK1/2 monoclonal antibody (Cell Signaling, Danvers, MA; clone no. 20G11, 1:150 for 2 hr at room temperature) in a Dako Autostainer Plus (Dako).

Techniques: Expressing, Diagnostic Assay

Journal: BMC Cancer

Article Title: Apoptosis inhibitor-5 overexpression is associated with tumor progression and poor prognosis in patients with cervical cancer

doi: 10.1186/1471-2407-14-545

Figure Lengend Snippet: Association of API5 and pERK1/2 expression in cervical cancer and CIN patients

Article Snippet: The slides were then stained with anti-API5 mouse monoclonal antibody (Sigma-Aldrich, clone no. 1C2, 1:250 for 2 hr at room temperature) and rabbit anti-pERK1/2 monoclonal antibody (Cell Signaling, Danvers, MA; clone no. 20G11, 1:150 for 2 hr at room temperature) in a Dako Autostainer Plus (Dako).

Techniques: Expressing

Journal: BMC Cancer

Article Title: Apoptosis inhibitor-5 overexpression is associated with tumor progression and poor prognosis in patients with cervical cancer

doi: 10.1186/1471-2407-14-545

Figure Lengend Snippet: Kaplan–Meier plots of disease-free survival (A - C) and overall survival (D - F) categorized based on API5 expression, pERK1/2 expression, and co-expression of API5/pERK1/2. High API5 expression associated with short disease-free survival (A , P = 0.001) and overall survival rate (D , P = 0.001). Patients with high pERK1/2 expression displayed worse overall survival (E , P = 0.040) . The association of high API5/pERK1/2 with disease-free survival (C) and overall survival (F) was significantly different from that of low API5/pERK1/2 ( P < 0.001 for both).

Article Snippet: The slides were then stained with anti-API5 mouse monoclonal antibody (Sigma-Aldrich, clone no. 1C2, 1:250 for 2 hr at room temperature) and rabbit anti-pERK1/2 monoclonal antibody (Cell Signaling, Danvers, MA; clone no. 20G11, 1:150 for 2 hr at room temperature) in a Dako Autostainer Plus (Dako).

Techniques: Expressing

Journal: BMC Cancer

Article Title: Apoptosis inhibitor-5 overexpression is associated with tumor progression and poor prognosis in patients with cervical cancer

doi: 10.1186/1471-2407-14-545

Figure Lengend Snippet: Univariate and multivariate analyses of the associations between prognostic variables and overall survival in 173 cases of cervical cancer

Article Snippet: The slides were then stained with anti-API5 mouse monoclonal antibody (Sigma-Aldrich, clone no. 1C2, 1:250 for 2 hr at room temperature) and rabbit anti-pERK1/2 monoclonal antibody (Cell Signaling, Danvers, MA; clone no. 20G11, 1:150 for 2 hr at room temperature) in a Dako Autostainer Plus (Dako).

Techniques: